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brefeldin a  (InvivoGen)


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    InvivoGen brefeldin a
    Brefeldin A, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/InvivoGen
    Average 94 stars, based on 40 article reviews
    brefeldin a - by Bioz Stars, 2026-02
    94/100 stars

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    <t>Brefeldin</t> <t>A</t> inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.
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    brefeldin a  (InvivoGen)
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    <t>Brefeldin</t> <t>A</t> inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.
    Brefeldin A, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    brefeldin a - by Bioz Stars, 2026-02
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    brefeldin a  (MedChemExpress)
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    MedChemExpress brefeldin a
    <t>Brefeldin</t> <t>A</t> inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.
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    Brefeldin A inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: Brefeldin A inhibits DPV infection in DEF cells . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with different concentrations of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hours post infection (hpi). The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C). (D) DEF cells were treated with different concentrations of Brefeldin A for 48 h, and then the cell viability was determined using the CCK-8 cell viability assay kit.

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Infection, Control, Fluorescence, Microscopy, Virus, CCK-8 Assay, Viability Assay

    Brefeldin A therapeutically inhibits DPV infection . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI, and 0.05 µg/mL Brefeldin A was added before, in-the-meanwhile (inter), and after infection, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hpi. The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C).

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: Brefeldin A therapeutically inhibits DPV infection . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI, and 0.05 µg/mL Brefeldin A was added before, in-the-meanwhile (inter), and after infection, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 24 hpi. The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C).

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Infection, Control, Fluorescence, Microscopy, Virus

    Brefeldin A inhibits DPV infection within 48 h . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with 0.05 µg/mL Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at the indicated time points of 24, 48, 72, and 96 h. The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C).

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: Brefeldin A inhibits DPV infection within 48 h . (A-C) DEF cells were infected with DPV-CHv-GFP at 1 MOI for 1 h, then treated with 0.05 µg/mL Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at the indicated time points of 24, 48, 72, and 96 h. The scale bar is 100 µm (A). The infected cells were collected and the copy number of the DPV gene was detected by Q-PCR (B), and the cell supernatant was collected to determine the virus titer TCID 50 (C).

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Infection, Control, Fluorescence, Microscopy, Virus

    The influence of Brefeldin A on the life cycle of DPV . (A-D) Brefeldin A was added at different stages of the viral life cycle, adsorption (A), endocytosis (B), replication (C), and release (D). For details, please refer to the materials and methods.

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: The influence of Brefeldin A on the life cycle of DPV . (A-D) Brefeldin A was added at different stages of the viral life cycle, adsorption (A), endocytosis (B), replication (C), and release (D). For details, please refer to the materials and methods.

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Adsorption

    Brefeldin A affects cell-to-cell spread of the DPV . (A and B) DEF cells were infected with DPV-CHv-GFP at 0.001 MOI for 1 h, then treated with 0.05 µg/mL or 0.1 µg/mL of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 36 hpi. The scale bar is 100 µm (A). The area of 10 virus fluorescent plaques in each group was randomly selected for statistical analysis, DMSO treatment group was set to 100%. (B).

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: Brefeldin A affects cell-to-cell spread of the DPV . (A and B) DEF cells were infected with DPV-CHv-GFP at 0.001 MOI for 1 h, then treated with 0.05 µg/mL or 0.1 µg/mL of Brefeldin A, DMSO was added as control. The viral fluorescence spots were photographed under fluorescence microscope at 36 hpi. The scale bar is 100 µm (A). The area of 10 virus fluorescent plaques in each group was randomly selected for statistical analysis, DMSO treatment group was set to 100%. (B).

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Infection, Control, Fluorescence, Microscopy, Virus

    Brefeldin A affects the distribution of DPV envelope proteins . (A and B) DEF cells were transfected with UL51-Flag or UL44-HA expression plasmid, and 0.05 µg/mL Brefeldin A was added 30 h after transfection, DMSO was added as control. Indirect immunofluorescence (IFA) analysis was performed 6 h after drug treatment, and the fluorescence signal was observed by confocal immunofluorescence microscope. The scale size is 10 µm.

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: Brefeldin A affects the distribution of DPV envelope proteins . (A and B) DEF cells were transfected with UL51-Flag or UL44-HA expression plasmid, and 0.05 µg/mL Brefeldin A was added 30 h after transfection, DMSO was added as control. Indirect immunofluorescence (IFA) analysis was performed 6 h after drug treatment, and the fluorescence signal was observed by confocal immunofluorescence microscope. The scale size is 10 µm.

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Transfection, Expressing, Plasmid Preparation, Control, Immunofluorescence, Fluorescence, Microscopy

    Brefeldin A affects the release of nucleocapsids and virion envelopment . (A) DEF cells were infected with DPV-CHv-GFP at 5 MOI for 2 h, cultured at 37°C for 12 h, and then treated with 0.05 µg/mL Brefeldin A for 6 h, DMSO was added as control. Cell samples were collected, centrifuged at 3000 r/min for 15 min at room temperature, and then fixed in 2.5% glutaraldehyde. After fixation, they were sent to Chengdu Lilai Biotechnology for electron microscope preparation and photography. The blue arrows indicate Golgi vesicle encapsulated virions and mature virions, and the red arrows indicate viral nucleocapsids and immature virions.

    Journal: Poultry Science

    Article Title: Brefeldin A inhibits duck plague virus replication by impairing virion envelopment

    doi: 10.1016/j.psj.2026.106509

    Figure Lengend Snippet: Brefeldin A affects the release of nucleocapsids and virion envelopment . (A) DEF cells were infected with DPV-CHv-GFP at 5 MOI for 2 h, cultured at 37°C for 12 h, and then treated with 0.05 µg/mL Brefeldin A for 6 h, DMSO was added as control. Cell samples were collected, centrifuged at 3000 r/min for 15 min at room temperature, and then fixed in 2.5% glutaraldehyde. After fixation, they were sent to Chengdu Lilai Biotechnology for electron microscope preparation and photography. The blue arrows indicate Golgi vesicle encapsulated virions and mature virions, and the red arrows indicate viral nucleocapsids and immature virions.

    Article Snippet: Brefeldin A (BFA) was purchased from MedChemExpress and diluted with dimethyl sulfoxide (DMSO), with a purity of 99.82%.

    Techniques: Infection, Cell Culture, Control, Microscopy